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d cycloserine  (Chem Impex International)


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    Chem Impex International d cycloserine
    D Cycloserine, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d+cycloserine/D-Cycloserine/pm41773869-98-23-24
    Average 96 stars, based on 2 article reviews
    d cycloserine - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Phylogenomic analysis of the collagen-like BclA proteins in Clostridioides difficile .
    Article Snippet: When needed, BHIS medium was supplemented with 15 μg mL−1 thiamphenicol (Sigma-Aldrich, USA), 16 μg mL−1 cefoxitin (Sigma-Aldrich, USA), and 250 μg mL−1 D-cycloserine (Chem-Impex International, USA).



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    IleRS-silenced (IleRS_KD) strains were compared with empty vector strains (PLJR962_KD) under four treatment conditions: untreated, PZA without ATC (no IleRS silencing), ATC alone (silencing control), and ATC + PZA. A Bar charts demonstrates the synergistic bactericidal effect of IleRS silencing and PZA on M. abscessus and M. marinum under acidic conditions (pH 5.0 and 5.5), with greater killing when ATC + PZA are combined than with either treatment alone ( n = 3). B In the NR medium (non-replicating stress model; see Methods), IleRS silencing further enhances PZA activity, indicating a synergistic interaction under stress ( n = 3). C alamarBlue microplate assay determining minimum inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RFP), ethambutol (EMB), pyrazinamide (PZA), amikacin (AMK), moxifloxacin (MXF), ethionamide (ETH), <t>D-cycloserine</t> <t>(DCS),</t> sodium para-aminosalicylic acid (PAS), linezolid (LZD), bedaquiline (BDQ), meropenem (MEM), clofazimine (CFZ), and clarithromycin (CLA). Drugs were tested at 0.004×–0.5× MIC. Empty vector strains and IleRS-silenced strains were tested with or without ATC induction. D Survival of M. abscessus and M. marinum in RAW 264.7 and THP-1 macrophage infected at MOI = 1. At 24 and 72 h post-infection, macrophages were washed, extracellular bacteria were removed with gentamicin (see Methods), cells were lysed with 0.01% SDS, and lysates were plated on 7H10 to quantify viable bacteria ( n = 3). Error bars represent mean ± SD. Statistical significance was determined by two-way ANOVA. Differences were considered statistically significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****). “ns” denotes no significant difference.
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    IleRS-silenced (IleRS_KD) strains were compared with empty vector strains (PLJR962_KD) under four treatment conditions: untreated, PZA without ATC (no IleRS silencing), ATC alone (silencing control), and ATC + PZA. A Bar charts demonstrates the synergistic bactericidal effect of IleRS silencing and PZA on M. abscessus and M. marinum under acidic conditions (pH 5.0 and 5.5), with greater killing when ATC + PZA are combined than with either treatment alone ( n = 3). B In the NR medium (non-replicating stress model; see Methods), IleRS silencing further enhances PZA activity, indicating a synergistic interaction under stress ( n = 3). C alamarBlue microplate assay determining minimum inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RFP), ethambutol (EMB), pyrazinamide (PZA), amikacin (AMK), moxifloxacin (MXF), ethionamide (ETH), <t>D-cycloserine</t> <t>(DCS),</t> sodium para-aminosalicylic acid (PAS), linezolid (LZD), bedaquiline (BDQ), meropenem (MEM), clofazimine (CFZ), and clarithromycin (CLA). Drugs were tested at 0.004×–0.5× MIC. Empty vector strains and IleRS-silenced strains were tested with or without ATC induction. D Survival of M. abscessus and M. marinum in RAW 264.7 and THP-1 macrophage infected at MOI = 1. At 24 and 72 h post-infection, macrophages were washed, extracellular bacteria were removed with gentamicin (see Methods), cells were lysed with 0.01% SDS, and lysates were plated on 7H10 to quantify viable bacteria ( n = 3). Error bars represent mean ± SD. Statistical significance was determined by two-way ANOVA. Differences were considered statistically significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****). “ns” denotes no significant difference.
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    IleRS-silenced (IleRS_KD) strains were compared with empty vector strains (PLJR962_KD) under four treatment conditions: untreated, PZA without ATC (no IleRS silencing), ATC alone (silencing control), and ATC + PZA. A Bar charts demonstrates the synergistic bactericidal effect of IleRS silencing and PZA on M. abscessus and M. marinum under acidic conditions (pH 5.0 and 5.5), with greater killing when ATC + PZA are combined than with either treatment alone ( n = 3). B In the NR medium (non-replicating stress model; see Methods), IleRS silencing further enhances PZA activity, indicating a synergistic interaction under stress ( n = 3). C alamarBlue microplate assay determining minimum inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RFP), ethambutol (EMB), pyrazinamide (PZA), amikacin (AMK), moxifloxacin (MXF), ethionamide (ETH), <t>D-cycloserine</t> <t>(DCS),</t> sodium para-aminosalicylic acid (PAS), linezolid (LZD), bedaquiline (BDQ), meropenem (MEM), clofazimine (CFZ), and clarithromycin (CLA). Drugs were tested at 0.004×–0.5× MIC. Empty vector strains and IleRS-silenced strains were tested with or without ATC induction. D Survival of M. abscessus and M. marinum in RAW 264.7 and THP-1 macrophage infected at MOI = 1. At 24 and 72 h post-infection, macrophages were washed, extracellular bacteria were removed with gentamicin (see Methods), cells were lysed with 0.01% SDS, and lysates were plated on 7H10 to quantify viable bacteria ( n = 3). Error bars represent mean ± SD. Statistical significance was determined by two-way ANOVA. Differences were considered statistically significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****). “ns” denotes no significant difference.
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    IleRS-silenced (IleRS_KD) strains were compared with empty vector strains (PLJR962_KD) under four treatment conditions: untreated, PZA without ATC (no IleRS silencing), ATC alone (silencing control), and ATC + PZA. A Bar charts demonstrates the synergistic bactericidal effect of IleRS silencing and PZA on M. abscessus and M. marinum under acidic conditions (pH 5.0 and 5.5), with greater killing when ATC + PZA are combined than with either treatment alone ( n = 3). B In the NR medium (non-replicating stress model; see Methods), IleRS silencing further enhances PZA activity, indicating a synergistic interaction under stress ( n = 3). C alamarBlue microplate assay determining minimum inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RFP), ethambutol (EMB), pyrazinamide (PZA), amikacin (AMK), moxifloxacin (MXF), ethionamide (ETH), D-cycloserine (DCS), sodium para-aminosalicylic acid (PAS), linezolid (LZD), bedaquiline (BDQ), meropenem (MEM), clofazimine (CFZ), and clarithromycin (CLA). Drugs were tested at 0.004×–0.5× MIC. Empty vector strains and IleRS-silenced strains were tested with or without ATC induction. D Survival of M. abscessus and M. marinum in RAW 264.7 and THP-1 macrophage infected at MOI = 1. At 24 and 72 h post-infection, macrophages were washed, extracellular bacteria were removed with gentamicin (see Methods), cells were lysed with 0.01% SDS, and lysates were plated on 7H10 to quantify viable bacteria ( n = 3). Error bars represent mean ± SD. Statistical significance was determined by two-way ANOVA. Differences were considered statistically significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****). “ns” denotes no significant difference.

    Journal: Communications Biology

    Article Title: Isoleucyl-tRNA synthetase depletion reveals vulnerabilities in Mycobacterium abscessus and Mycobacterium marinum

    doi: 10.1038/s42003-025-08841-y

    Figure Lengend Snippet: IleRS-silenced (IleRS_KD) strains were compared with empty vector strains (PLJR962_KD) under four treatment conditions: untreated, PZA without ATC (no IleRS silencing), ATC alone (silencing control), and ATC + PZA. A Bar charts demonstrates the synergistic bactericidal effect of IleRS silencing and PZA on M. abscessus and M. marinum under acidic conditions (pH 5.0 and 5.5), with greater killing when ATC + PZA are combined than with either treatment alone ( n = 3). B In the NR medium (non-replicating stress model; see Methods), IleRS silencing further enhances PZA activity, indicating a synergistic interaction under stress ( n = 3). C alamarBlue microplate assay determining minimum inhibitory concentrations (MICs) of isoniazid (INH), rifampicin (RFP), ethambutol (EMB), pyrazinamide (PZA), amikacin (AMK), moxifloxacin (MXF), ethionamide (ETH), D-cycloserine (DCS), sodium para-aminosalicylic acid (PAS), linezolid (LZD), bedaquiline (BDQ), meropenem (MEM), clofazimine (CFZ), and clarithromycin (CLA). Drugs were tested at 0.004×–0.5× MIC. Empty vector strains and IleRS-silenced strains were tested with or without ATC induction. D Survival of M. abscessus and M. marinum in RAW 264.7 and THP-1 macrophage infected at MOI = 1. At 24 and 72 h post-infection, macrophages were washed, extracellular bacteria were removed with gentamicin (see Methods), cells were lysed with 0.01% SDS, and lysates were plated on 7H10 to quantify viable bacteria ( n = 3). Error bars represent mean ± SD. Statistical significance was determined by two-way ANOVA. Differences were considered statistically significant at P < 0.05 (*), P < 0.01 (**), P < 0.001 (***), and P < 0.0001 (****). “ns” denotes no significant difference.

    Article Snippet: All antibiotics used in this study—including isoniazid (INH), rifampicin (RFP), ethambutol (EMB), pyrazinamide (PZA), amikacin (AMK), moxifloxacin (MXF), ethionamide (ETH), D-cycloserine (DCS), sodium para-aminosalicylic acid (PAS), linezolid (LZD), bedaquiline (BDQ), meropenem (MEM), clofazimine (CFZ), doxycycline (DOX) and clarithromycin (CLA)—were obtained from Sangon Biotech (Shanghai, China).

    Techniques: Plasmid Preparation, Control, Activity Assay, Infection, Bacteria